| Primary Identifier | MGI:6316341 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Frmd3 |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | IMPC |
| molecularNote | This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences TAAGCGGCAAAAGATCACAG and CTCCACAGCCTACTTCCTGG, which resulted in a 227 bp deletion beginning at Chromosome 4 position 74,119,755 bp and ending after 74,119,981 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000398544 (exon 4) and 148 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 98 and early truncation 73 amino acids later. |
