| First Author | Bresser K | Year | 2020 |
| Journal | Commun Biol | Volume | 3 |
| Issue | 1 | Pages | 273 |
| PubMed ID | 32472011 | Mgi Jnum | J:293819 |
| Mgi Id | MGI:6453712 | Doi | 10.1038/s42003-020-0979-0 |
| Citation | Bresser K, et al. (2020) A mouse model that is immunologically tolerant to reporter and modifier proteins. Commun Biol 3(1):273 |
| abstractText | Reporter proteins have become an indispensable tool in biomedical research. However, exogenous introduction of these reporters into mice poses a risk of rejection by the immune system. Here, we describe the generation, validation and application of a multiple reporter protein tolerant 'Tol' mouse model that constitutively expresses an assembly of shuffled reporter proteins from a single open reading frame. We demonstrate that expression of the Tol transgene results in the deletion of CD8(+) T cells specific for a model epitope, and substantially improves engraftment of reporter-gene transduced T cells. The Tol strain provides a valuable mouse model for cell transfer and viral-mediated gene transfer studies, and serves as a methodological example for the generation of poly-tolerant mouse strains. |
