Primary Identifier | MGI:6343327 | Allele Type | Targeted |
Attribute String | Epitope tag, Inserted expressed sequence | Gene | Col1a1 |
Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
Induced With | doxcycline/tetracycline | Is Recombinase | false |
Is Wild Type | false |
molecularNote | ES cells containing the Gt(ROSA)26Sortm1(rtTA2S-M2)Jae mutation were retargeted. Cells were injected with a "flip-in plasmid" containing a tetracycline responsive element (TRE or tetO) and mouse Nup88 cDNA sequence with an HA tag and a Flpe-recombinase plasmid to facilitate tetO-Oct4 integration into the 3' UTR region of Col1a1. |