| Primary Identifier | MGI:6355366 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Stx4a |
| Transmission | Germline | Strain of Origin | C57BL/6J |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site was inserted upstream of exon 1 (GRCm38; Chr7:127841278) and a second loxP site, followed by the phosphoglucokinase promoter-driven neomycin resistance gene (PGK-Neo) flanked by two FRT sites, was introduced in intron 4 (GRCm38; Chr7:127842997). The neomycin selection cassette was removed via Flp-mediated recombination, leaving exons 1-4 floxed. Subsequent Cre expression in the germline resulted in the deletion of the loxP-flanked genomic sequences, generating a knockout allele. |
