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Allele : Tg(Acta2-GCaMP8.1/mVermilion)B34-4Mik transgene insertion B34-4, Michael I Kotlikoff
Primary Identifier  MGI:6324941 Allele Type  Transgenic
Attribute String  Reporter Gene  Tg(Acta2-GCaMP8.1/mVermilion)B34-4Mik
Strain of Origin  C57BL/6J x SJL/J Is Recombinase  false
Is Wild Type  false
molecularNote  Acta2-GCaMP8.1-mVermilion transgenic mice (also called Acta2-GCaMP8.1/mVermilion or Acta2-GCaMP8.1-mVermilion) were designed in the laboratory of Dr. Michael I. Kotlikoff (Cornell University) as part of Cornell/National Heart Lung Blood Resource for Optogenetic Mouse Signaling (CHROMus). The ~175 kbp C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-370F21 was obtained; containing the entire actin alpha 2 locus (Acta2) as well as ~92 kbp of 5' flanking sequences (including the complete Fas locus) and ~68 kbp of 3' flanking sequences (including the complete Stambpl1 locus). Using homologous recombination/BAC recombineering, a 2993 bp GCaMP8.1/mVermilion-SV40pA construct (see details below) was inserted into the ATG start site of the BAC Acta2 gene (replacing the initiation codon of Acta2 in exon 2). No other loci on the BAC were altered. The 11,612 bp BAC vector (pBACe3.6) contained a chloramphenicol selection cassette.
  • mutations:
  • Insertion
  • synonyms:
  • B34-Acta2<BAC>-GCaMP8.1_mVermilion,
  • B34-Acta2<BAC>-GCaMP8.1_mVermilion
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1 Feature

Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

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