|  Help  |  About  |  Contact Us

Allele : Tg(Acta2-GCaMP8.1/mVermilion)B34-4Mik transgene insertion B34-4, Michael I Kotlikoff
Primary Identifier  MGI:6324941 Allele Type  Transgenic
Attribute String  Reporter Gene  Tg(Acta2-GCaMP8.1/mVermilion)B34-4Mik
Strain of Origin  C57BL/6J x SJL/J Is Recombinase  false
Is Wild Type  false
molecularNote  Acta2-GCaMP8.1-mVermilion transgenic mice (also called Acta2-GCaMP8.1/mVermilion or Acta2-GCaMP8.1-mVermilion) were designed in the laboratory of Dr. Michael I. Kotlikoff (Cornell University) as part of Cornell/National Heart Lung Blood Resource for Optogenetic Mouse Signaling (CHROMus). The ~175 kbp C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-370F21 was obtained; containing the entire actin alpha 2 locus (Acta2) as well as ~92 kbp of 5' flanking sequences (including the complete Fas locus) and ~68 kbp of 3' flanking sequences (including the complete Stambpl1 locus). Using homologous recombination/BAC recombineering, a 2993 bp GCaMP8.1/mVermilion-SV40pA construct (see details below) was inserted into the ATG start site of the BAC Acta2 gene (replacing the initiation codon of Acta2 in exon 2). No other loci on the BAC were altered. The 11,612 bp BAC vector (pBACe3.6) contained a chloramphenicol selection cassette.
  • mutations:
  • Insertion
  • synonyms:
  • B34-Acta2<BAC>-GCaMP8.1_mVermilion,
  • B34-Acta2<BAC>-GCaMP8.1_mVermilion
Paste the following link
Quick Links:
SummaryGenomePhenotypeOther
 
Quick Links:
SummaryGenomePhenotypeOther
 
Lists
This Allele isn't in any lists. Upload a list.

1 Feature

Trail: Allele

Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

Other

1 Carried By

Trail: Allele

0 Driven By

3 Publication categories

Trail: Allele




MouseMine is a collaboration between MGI at The Jackson Laboratory and the InterMine project at the Cambridge Systems Biology Centre. MouseMine is funded through a grant from the NIH/NHGRI.The Jackson Laboratory makes no representation about the suitability or accuracy of this software or data for any purpose, and makes no warranties, either express or implied, including merchantability and fitness for a particular purpose or that the use of this software or data will not infringe any third party patents, copyrights, trademarks, or other rights. the software and data are provided "as is". This software and data are provided to enhance knowledge and encourage progress in the scientific community and are to be used only for research and educational purposes. Any reproduction or use for commercial purpose is prohibited without the prior express written permission of the Jackson Laboratory.

Copyright © 2017 by The Jackson Laboratory. All Rights Reserved

© 2002 - 2017 Department of Genetics, University of Cambridge, Downing Street,
Cambridge CB2 3EH, United Kingdom