Primary Identifier | MGI:6382286 | Allele Type | Targeted |
Attribute String | Recombinase | Gene | Slc6a5 |
Transmission | Germline | Strain of Origin | C57BL/6N |
Is Recombinase | true | Is Wild Type | false |
molecularNote | A Cre cDNA, a polyadenylation signal (pA) from the human granulocyte-colony stimulating factor gene, a phosphoglycerate kinase (PGK) promoter-driven neomycin resistance gene (neo)-pA cassette (PGK-neo-pA cassette) flanked by two frt sites were inserted into the translation initiation site (in exon 2) of the mouse Slc6a5 gene by homologous recombination in ES cells. The PGK-neo-pA cassette was removed by crossing mice with FLP66 transgenic mice. |