| Primary Identifier | MGI:6356325 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Pacc1 |
| Strain of Origin | C57BL/6 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | CRISPR/Cas9 mutagenesis was used to insert two loxP sites, one 397 bp upstream of exon 2 and a second 309 bp dpwnstream of exon 2. Cre-mediated recombination deleted exon 2, resulting in a frameshift mutation before the TM1 domain and creating a null allele. qPCR analysis confirmed the absence of mRNA expression in homozygous mutant brains. |
