| Primary Identifier | MGI:6356401 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Stk38l |
| Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | KOMP-CSD |
| molecularNote | The L1L2_Bact_P cassette was inserted at position 146669464 of Chromosome 6 upstream of the critical exon 7 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 7 at position 146670075. The critical exon 7 is thus flanked by loxP sites. The lacZ open reading frame is not in frame with exon 6 and not expected to produce a reporter protein (confirmed by immunoblotting). Cre expression without flp expression generated a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml |
