| First Author | Liu C | Year | 2023 |
| Journal | Nucleic Acids Res | Volume | 51 |
| Issue | 14 | Pages | 7357-7375 |
| PubMed ID | 37378420 | Mgi Jnum | J:355465 |
| Mgi Id | MGI:7517653 | Doi | 10.1093/nar/gkad524 |
| Citation | Liu C, et al. (2023) RNase H1 facilitates recombinase recruitment by degrading DNA-RNA hybrids during meiosis. Nucleic Acids Res 51(14):7357-7375 |
| abstractText | DNA-RNA hybrids play various roles in many physiological progresses, but how this chromatin structure is dynamically regulated during spermatogenesis remains largely unknown. Here, we show that germ cell-specific knockout of Rnaseh1, a specialized enzyme that degrades the RNA within DNA-RNA hybrids, impairs spermatogenesis and causes male infertility. Notably, Rnaseh1 knockout results in incomplete DNA repair and meiotic prophase I arrest. These defects arise from the altered RAD51 and DMC1 recruitment in zygotene spermatocytes. Furthermore, single-molecule experiments show that RNase H1 promotes recombinase recruitment to DNA by degrading RNA within DNA-RNA hybrids and allows nucleoprotein filaments formation. Overall, we uncover a function of RNase H1 in meiotic recombination, during which it processes DNA-RNA hybrids and facilitates recombinase recruitment. |
