| Primary Identifier | MGI:6356625 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Rbks |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | EUCOMM |
| description | Cell line EPD0116_3_A10 was successfully used to make chimeric mice. Germline transmission was accomplished. J:236053 |
| molecularNote | The L1L2_Bact_P cassette was inserted at position 31830399 of Chromosome 5 upstream of the critical exon (exon 2) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon at position 31831256. Exon 2 is thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and exon 2. RT-PCR analysis confirmed deletion of exon 2 at the RNA level. |
