| Primary Identifier | MGI:6356819 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Rtbdn |
| Transmission | Germline | Strain of Origin | (C57BL/6NTac x 129S6/SvEvTac)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A 2.64kb eGFP cassette (eGFP-LoxP-FRT-Neo-FRT-LoxP) replaced 5.72Kb of genomic sequence, which flanks the Rtbdn start (ATG) codon and stop (TAG) codon. The neomycin selection cassette was removed via FLP-mediated recombination in the germline. Western blot analysis using an anti-Rtbdn antibody confirmed the absence of RTBDN protein expression in neural retina lysates from homozygous mutant mice. eEGFP expression was found exclusively in the neural retina and absent from all other tissues tested. Immunofluorescence revealed no co-localization of cone arrestin with native eGFP, suggesting that the Rtbdn promoter is not active in cones and it is specific to rod photoreceptors. |
