| Primary Identifier | MGI:6370001 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Phf8 |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exons 7 and 8, encoding the code region of the JmjC demethylase domain, were flanked by loxP sites. A FRT-flanked neomycin resistance gene was inserted downstream of exon 8 before the second loxP site. Cre-mediated recombination deleted exons 7 and 8 and the selection cassette in the germline. Western blot and immunostaining on brains confirmed absence of protein. |
