| Primary Identifier | MGI:6358590 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Serpina1 |
| Strain of Origin | C57BL/6J | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | CRISPR/Cas9 technology is used to disrupt five highly homologous gene paralogs through the targeting of exon 2 using a combination of four gRNAs, deleting 74 kb (103822963-103897826), resulting in the excision of Serpina1a and 1c. Null status was confirmed by transcriptome analysis. Western blot confirmed absence of the alpha1-antitrypsin protein in serum. |
