| Primary Identifier | MGI:6358591 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Serpina1 |
| Strain of Origin | C57BL/6J | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | CRISPR/Cas9 technology is used to disrupt five highly homologous gene paralogs through the targeting of exon 2 using a combination of four gRNAs. The population analyzed for phenotype contained individuals with alleles from the 3 lines (7A, 24B, 31C) and includes Serpina1em1Chmu, Serpina1em2Chmu, and Serpina1em2Chmu. The pound symbol (#) is used when no line is specified or lines are pooled. Western blot and immunohistochemistry confirmed absence of the alpha1-antitrypsin protein in serum and liver tissue, respectively. |
