Primary Identifier | MGI:6384614 | Allele Type | Endonuclease-mediated |
Attribute String | Inducible, Recombinase | Gene | Slc17a8 |
Strain of Origin | C57BL/6N | Induced With | tamoxifen |
Is Recombinase | true | Is Wild Type | false |
molecularNote | A plasmid encoding a guide RNA and PAM sequence TGG was designed to introduce a P2A peptide fused to an icre/ERT2 cassette into exon 12, immediately before the stop codon, of the solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 8 (Slc17a8) gene. Guide RNA and the cas9 nuclease were introduced into the cytoplasm of C57BL/6N-derived fertilized eggs with well recognized pronuclei. |