Primary Identifier | MGI:6367594 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Mirc33 |
Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
Is Recombinase | false | Is Wild Type | false |
Project Collection | Sanger miRNA knockouts |
molecularNote | Mir29a and Mir29b1 in this microRNA cluster were replaced via homologous recombination with a puromycin/thymidine kinase fusion gene under the control of EM7/PGK promoters. The inserted selection cassette was flanked by loxP and F3/FRT sites. Cre-mediated recombination excised the replacement cassette. |