| Primary Identifier | MGI:6384859 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Isy1 |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | IMPC |
| molecularNote | This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences ATGAGGCATTCAAAGCCTGG and TGTATTGTGAACAAAAGAGC, which resulted in a 431 bp deletion beginning at Chromosome 6 position 87,825,195 bp and ending after 87,825,625 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000323874 (exon 7) and 313 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 100 and early truncation 12 amino acids later. |
