| Primary Identifier | MGI:6388407 | Allele Type | Targeted |
| Attribute String | Conditional ready, Inducible, Recombinase, Reporter | Gene | Gt(ROSA)26Sor |
| Transmission | Germline | Strain of Origin | Not Specified |
| Induced With | tamoxifen | Is Recombinase | true |
| Is Wild Type | false |
| molecularNote | A Rosa-CAG targeting vector was designed to insert cassette containing (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), loxP-site, Dre recombinase fused with a mutant form of estrogen receptor hormon-binding domaine C (ERT2), STOP cassette (with stop codons in all 3 reading frames and a triple polyA signal), second loxP-site, ZsGreen sequence (an enhanced green fluorescent protein engineered for brighter fluorescence and higher expression in mammalian cells), WPRE (woodchuck hepatitis virus posttranscriptional regulatory element)-polyA, and Frt flanked neomicyne selection cassette (CAG-loxp-DreERT2-stop-loxp-ZsGreen-polyA-Frt-Neo-Frt) into the Rosa26 locus. |
