| Primary Identifier | MGI:6368160 | Allele Type | Targeted |
| Attribute String | Inducible, Inserted expressed sequence | Gene | Col1a1 |
| Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
| Induced With | doxycycline/tetracycline | Is Recombinase | false |
| Is Wild Type | false |
| description | The allele was made in KH2 cells that carry Gt(ROSA)26Sortm1(rtTA*M2)Jae and Col1a1tm13(neo/hygro*)Jae. |
| molecularNote | The targeting vector is designed to insert an FRT-flanked PGK-Neo-pA 500 bp downstream of the 3' UTR of the Col1a1 locus. The FRT-flanked PGK-Neo-pA in the 3' UTR of the Col1a1 locus is then replaced by co-injection of a mutant tetO-H3.3f3a plasmid and flpe recombinase. The plasmid contains a splice acceptor-double polyA sequence and the tetracycline responsive element (tetO) upstream of the H3.3 histone A gene carrying a lysine-to-methionine substitution at amino acid 9 (K9M). |
