| Primary Identifier | MGI:6385663 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Anapc7 |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | IMPC |
| molecularNote | This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences ATATTAGGAGGTGTTTTGCG and AACTATGGTTAGATAGAGCA, which resulted in a 427 bp deletion beginning at Chromosome 5 position 122,432,528 bp and ending after 122,432,954 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000189114 (exon 5) and 315 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 136 and early truncation 6 amino acids later. |