Primary Identifier | MGI:6368179 | Allele Type | Targeted |
Attribute String | Null/knockout, Reporter | Gene | Rrh |
Transmission | Germline | Strain of Origin | Not Specified |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Using homologous recombination in ES cells, a targeting vectir replaced a 4.3-kb fragment starting at the initiator methionine in exon 1 and a part of intron 1 of a mouse Rrh genomic fragment with a LacZ-coding region followed by a neomycin resistance cassette (PGK-Neo). Immunoblot analysis on retinal pigment epithelium (RPE) homogenates and immunohistochemistry of eyecup sections confirmed absence of protein in homozygous mice. It is a null allele. |