| Primary Identifier | MGI:6401218 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Rhoa |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exon 3 was replaced with a minigene containing Rhoa exons 3 to 6 flanked by loxP sites followed by a neomycin selection cassette and a mutant exon 3 encoding a glycine to valine substitution at amino acid 17. The selection cassette was removed via Flp-mediated recombination. In the absence of cre recombinase, exon 2 is spliced into exons 3-6 minigene resulting in expression of a wild-type mRNA. In the presence of cre recombinase, the floxed exon 3-6 minigene is removed and the mutant G17V allele is expressed. |
