| Primary Identifier | MGI:6392829 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change, Reporter | Gene | Gt(ROSA)26Sor |
| Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6NCrl)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The Ai27 targeting construct was modified to contain: CAG promoter, FRT site, loxP site, stop codons, three polyadenylation sequeces, loxP site, FRET-based full-length vinculin tension sensor (vinculin head, monomeric teal fluorescent protein1 mTFP1, spacer, Venus reporter gene and vinculin tail), WPRE, bovine growth hormone polyadenylation sequence, attB site, PGK promoter, FRT site, neomycin resistance cassette and attP site. The entire sequence was inserted between exons 1 and 2. |
