Primary Identifier | MGI:6423503 | Allele Type | Targeted |
Attribute String | Inducible, Reporter | Gene | Hprt1 |
Transmission | Germline | Strain of Origin | 129S5/SvEvBrd |
Induced With | doxycycline/tetracycline | Is Recombinase | false |
Is Wild Type | false |
description | ES cell line = a mutant A17 line (A2Lox.cre) that carries a doxycycline-inducible Cre recombinase ORF flanked by loxP and lox2272 sites, upstream of a non-functional neomycin resistance (neo) gene. |
molecularNote | The tetracycline promoter drives inducible expression along with a ubiquitous CMV of the fluorescent timer protein with the medium mutations (mCherry*M23E, N28D, T48S, K75R, L88W, M155I, Q193L, L204M, Y219C, and R225H) fused to human H2BC9 protein for chromatin localization. |