Primary Identifier | MGI:6423628 | Allele Type | Targeted |
Attribute String | Constitutively active, Epitope tag | Gene | Stk39 |
Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A loxP flanked PGK-neomycin selection cassette and an HA-tagged Stk39 cDNA containing two mutations, a threonine to glutamate change at residue 243 (T243E) and a serine to aspartate change at 383 (S383D), and an in-frame stop codon were inserted after the start codon of Stk39. Cre-mediated recombination excised the selection cassette resulting in expression of the constitutively active kinase under the control of the native gene promoter. |