| Primary Identifier | MGI:6438148 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Dmd |
| Strain of Origin | C57BL/6 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | CRISPR/Cas9 technology generated a 908 bp deletion that eliminated exon 44, producing a premature stop codon in exon 45. This is one of the most common deletions responsible for Duchenne muscular dystrophy in humans. RT-PCR and product sequencing confirmed the deletion of exon 44. Western blot analysis confirmed the absence of protein. |
