| Primary Identifier | MGI:6407110 | Allele Type | Targeted |
| Attribute String | Humanized sequence, Inserted expressed sequence | Gene | Cbfb |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exon 5 was replaced by a construct composed of the first 25 bp of mouse exon 5 fused to the 3' end of the human CBFB/MYH11 cDNA (resulting from a chromosome 16 inversion common in human acute myeloid leukemia subtype M4Eo) containing mutated six charged residues in helices D and E to threonine, serine, or alanine residues: D (NANRRKL to NSNRASL) and E (QRELDEA to QAELTSA). The human cDNA fragment used contained the last 66 bp of human exon 5 and exons 33-42 of MYH11. A bovine growth hormone polyadenylation site and a neomycin cassette were inserted immediately downstream. Western blot confirmed expression of the fusion protein. |
