| Primary Identifier | MGI:6435110 | Allele Type | Targeted |
| Attribute String | Not Specified | Gene | Igh |
| Transmission | Germline | Strain of Origin | B6.Cg-Thy1<a> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Within the VH186.2-DFL16.1-JH2 sequence of the genomic DNA of B1-8hi mice, the Leu33 TTG codon was changed to TGG (Trp) and the B1-8 germline fragment was subcloned into a targeting vector which was electroporated into Bruce4 ES cells to generate homologous recomination. After generation of chimeric mice, the neomycin-resistance gene (neor) was removed by crossing to CAG-Flp transgenic mice. |
