| Primary Identifier | MGI:6446938 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Klf5 |
| Transmission | Germline | Strain of Origin | C57BL/6 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The targeting vector is designed to insert, from 5' to 3', a loxP site, exons 2-4 of the gene, a STOP cassette, an FRT-flanked neomycin resistance cassette, and a second loxP site downstream of the endogenous exon 1 of the Klf5 gene. Endogenous exon 2 was modified to harbor a mutation at codon 358, resulting in a lysine to arginine mutation (K358R). Flp-mediated recombination removed the FRT-flanked neo cassette. |
