| First Author | Roh JI | Year | 2018 |
| Journal | Exp Anim | Volume | 67 |
| Issue | 2 | Pages | 229-237 |
| PubMed ID | 29343656 | Mgi Jnum | J:280224 |
| Mgi Id | MGI:6364403 | Doi | 10.1538/expanim.17-0123 |
| Citation | Roh JI, et al. (2018) CRISPR-Cas9-mediated generation of obese and diabetic mouse models. Exp Anim 67(2):229-237 |
| abstractText | Mouse models of obesity (ob/ob) and diabetes (db/db) in which the leptin (Lep) and leptin receptor (Lepr) genes have been mutated, respectively, have contributed to a better understanding of human obesity and type 2 diabetes and to the prevention, diagnosis, and treatment of these metabolic diseases. In this study, we report the first CRISPR-Cas9-induced Lep and Lepr knockout (KO) mouse models by co-microinjection of Cas9 mRNA and sgRNAs that specifically targeted Lep or Lepr in C57BL/6J embryos. Our newly established Lep and Lepr KO mouse models showed phenotypic disorders nearly identical to those found in ob/ob and db/db mice, such as an increase in body weight, hyperglycemia, and hepatic steatosis. Thus, Cas9-generated Lep and Lepr KO mouse lines will be easier for genotyping, to maintain the lines, and to use for future obesity and diabetes research. |
