Primary Identifier | MGI:6450045 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Obsl1 |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A loxP site was inserted upstream of exon 1, and a second loxP site followed by an FRT-flanked PGK-neo cassette was inserted downstream of exon 4. The neomycin selection cassette was removed via Flp-mediated recombination in the germline, and exon 4 was deleted via Cre-mediated recombination. |