Primary Identifier | MGI:6451472 | Allele Type | Targeted |
Attribute String | Null/knockout, Recombinase, Reporter | Gene | Oprm1 |
Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6NCrl)F1 |
Is Recombinase | true | Is Wild Type | false |
Project Collection | Sanger miRNA knockouts |
molecularNote | A cassette encoding Cre:GFP (a Myc-tag and nuclear localization signals at the N-terminus of Cre recombinase, which is fused to green fluorescent protein followed by a SV40 polyadenylation sequence) was inserted just 5' of the initiation codon in the first coding exon of the Oprm1 gene replacing the exon 1 via homologous recombination. The neomycin cassette was excised of through crosses with animals broadly expressing Flp recombinase. |