| Primary Identifier | MGI:6457549 | Allele Type | Targeted |
| Attribute String | Epitope tag | Gene | Gli3 |
| Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Sequence for triple FLAG tags was inserted immediately downstream of the ATG translation start site, followed by sequence for an AviTag (GLNDIFEAQKIEWHE) and GSG linker sequence. The peptide encoded by this allele is thus tagged with N-terminal FLAG tags and contains an N-terminal biotinylation site that allows for streptavidin capture. The loxP site flanked neomycin resistance gene cassette that was also inserted was removed through subsequent cre-mediated recombination using mice carrying the
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