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Allele : Tmie<em2Mll> transmembrane inner ear; endonuclease-mediated mutation 2, Ulrich Muller

Primary Identifier  MGI:6452851 Allele Type  Endonuclease-mediated
Attribute String  Epitope tag Gene  Tmie
Strain of Origin  C57BL/6J Is Recombinase  false
Is Wild Type  false
molecularNote  Using CRISPR/Cas9 technology, sequence 5'-GGCGGCAGCGGCGAGCAGAAACTCATCTCTGAAGAAGATCTGGAACAAAAGTTGATTTCAGAAGAAGATC TGGAACAGAAGCTCATCTCTGAGGAAGATCTG-3', encoding GGSGEQKLISEEDLEQKLISEEDLEQKLISEEDL, was inserted immediately before the endogenous stop codon, providing the encoded peptide with three copies of a MYC tag linked via a 4 amino-acid linker to the C-terminus.
  • mutations:
  • Insertion
  • synonyms:
  • Tmie-3X-MYC,
  • Tmie-3X-MYC
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1 Feature

Trail: Allele

Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

Other

0 Carried By

0 Driven By

2 Publication categories

Trail: Allele