| Primary Identifier | MGI:6466590 | Allele Type | Targeted |
| Attribute String | Humanized sequence | Gene | Pak3 |
| Transmission | Germline | Strain of Origin | 129S2/SvPas |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A C to T (CGC>TGC) change (g.143709671C>T) resulting in an arginine to cysteine substitution at amino acid 67 was introduced in the second coding exon. A loxP flanked neomycin resistance gene was inserted downstream of exon 2. Cre-mediated recombination removed the selection cassette. RT-PCR and Western blot analysis confirmed mRNA and protein expression in the brain. |
