| Primary Identifier | MGI:6469363 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Sdr9c7 |
| Strain of Origin | C57BL/6J | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | A crRNA (5â²-CAACCAGTTGTTTGGCCAAC-3â²) targeting exon 1 and a trans-activating crRNA (tra-crRNA) were used with CRISPR/Cas9 technology to create a knockout allele. An ssODN (5â²-TCTTCATCACTGGCTGTGACTCCGGCTTTGGGAATCTGTTGGCCAAACAACTGGTTGATAGGGGCATGAAAGTGCTTGCT-3â²) was included to include a silent (synonymous) c.117C>T mutation to avoid cleavage by Cas9, reducing the chances of creating potentially embryonic lethal bi-allelic mutations. The resulting mutation is a single basepair deletion (c.123delG) in exon 1. Lack of protein expression from this allele was confirmed by immunohistochemistry. |
