| Primary Identifier | MGI:6470699 | Allele Type | Targeted |
| Attribute String | Epitope tag | Gene | Neurog3 |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The targeting vector contains a 5.5 kb 5â arm and a 3.5 kb 3âarm. Lox71 and Lox2272 sites were inserted flanking the gene, where the cDNA was replaced with that encoding for an HA-tagged Ngn3 protein. The vector also contains a FRT-flanked Pgk-Puro-deltaTK cassette for positive selection by puromycin (targeting events) and negative selection by ganciclovir (RMCE events). |
