| Primary Identifier | MGI:6470718 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Pfkfb2 |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The phospho-acceptor sites Ser468 and Ser485 located in exon 15 were mutated to alanine (S468A and S485A). A wild-type copy of exon 15 floxed by loxP sites was inserted into intron 14-15 upstream of the exon 15 splice acceptor followed by a polyadenylation signal. A FRT-flanked neomycin selection cassette was inserted into intron 14-15. FlpE-mediated recombination removed the neo cassette. |
