| Primary Identifier | MGI:6470720 | Allele Type | Targeted |
| Gene | Pfkfb2 | Transmission | Germline |
| Strain of Origin | Not Specified | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | The phospho-acceptor sites Ser468 and Ser485 located in exon 15 were mutated to alanine (S468A and S485A). A wild-type copy of exon 15 floxed by loxP sites was inserted into intron 14-15 upstream of the exon 15 splice acceptor followed by a polyadenylation signal. A FRT-flanked neomycin selection cassette was inserted into intron 14-15. FlpE-mediated recombination removed the neo cassette. Cre-mediated recombination removed the wild-type exon 15 so the mutant protein is expressed. |
