Primary Identifier | MGI:6470589 | Allele Type | Targeted |
Attribute String | Epitope tag, Reporter | Gene | Ptf1a |
Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Exon 1 was replaced by the tdTomato reporter gene, a Ptf1a coding sequence with an N-terminal epitope tagged Strep/Flag (NSF) fusion, and an FRT-flanked hygro cassette via RMCE using the loxed cassette acceptor (LCA) allele-containing ES cells. Flp-mediated recombination removed the selection cassette. |