| Primary Identifier | MGI:6507851 | Allele Type | Targeted |
| Attribute String | Conditional ready, Inserted expressed sequence | Gene | Igs2 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| description | This allele was produced in zygotes collected from mice carrying either the Igs2tm3.1Luo allele or the Igs2tm4Luo allele (not specified which is used), which was generated in (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 ES cells. |
| molecularNote | Site-specific PhiC31 integrase-mediated transgenesis is used to insert the targeting construct containing an attB site, the CAG promoter, a loxP flanked PGK-neomycin STOP cassette, a FLAG-tagged mouse Met cDNA, a polyA signal and a second attB site into the intergenic site 2 locus. This construct was microinjected, along with the PhiC31 integrase mRNA into the pronuclei of zygotes of an FVB/N mouse that contains an attP docking site at the H11 locus which is either the Igs2tm3.1Luo or the Igs2tm4Luo. The PhiC31 integrase catalyzes recombination between the attB and attP sites. |
