Primary Identifier | MGI:6508396 | Allele Type | Targeted |
Attribute String | Conditional ready | Gene | Tpsg1 |
Transmission | Germline | Strain of Origin | C57BL/6NTac |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A loxP site was placed 1.9 kb upstream of the first exon. A second loxP site was placed in the first intron. A puromycin resistance (PuroR) gene flanked by F3 sites was inserted into intron 1 upstream of the second loxP site for positive selection. The puromycin resistance (PuroR) gene was removed via flp1-mediated recombination in the germline. |