| Primary Identifier | MGI:6690858 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change | Gene | Eaf1 |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Using a recombineering approach, a loxP site flanked neomycin resistance gene was inserted into intron 1. An FRT site flanked neo cassette and a loxP site were inserted into intron 2. The two neo cassettes were removed through subsequent cre-mediated and flp-mediated recombination, leaving a conditional-ready allele with exon 2 floxed. |
