| Primary Identifier | MGI:6729714 | Allele Type | Endonuclease-mediated |
| Attribute String | Recombinase | Gene | Atoh1 |
| Strain of Origin | C57BL/6N | Is Recombinase | true |
| Is Wild Type | false |
| molecularNote | CRISPR/cas9 genome editing is used to insert a P2A, a FlpO recombinase gene after the last amino acid codon and before the stop codon of Atoh1. The cassette contained a minor mutation at the end of FLPo (the last isoleucine amino acid was changed to a serine), which could have occurred possibly because of an imprecise DNA repair event. But, this amino acid change did not affect the enzymatic function of FLPo. |
