| Primary Identifier | MGI:6718511 | Allele Type | Targeted |
| Attribute String | Conditional ready, Inserted expressed sequence, Reporter | Gene | Gt(ROSA)26Sor |
| Transmission | Germline | Strain of Origin | 129 |
| Is Recombinase | false | Is Wild Type | false |
| description | The version of this allele that contains/ does not contain the neomycin selection cassette, Gt(ROSA)26Sortm5(CAG-Sun1/sfGFP)Nat, has been analyzed. |
| molecularNote | A targeting vector containing (from 5' to 3') a CAG promoter, a loxP site flanked STOP cassette, the mouse Sun1 cDNA fused with 2 copies of the superfolder GFP (sfGFP) green fluorescent protein gene and 6 copies of the Myc tag, and an FRT site flanked neomycin resistance gene cassette was inserted into the locus. Flp-mediated recombination removed the FRT-flanked neo cassette. Removal of the floxed stop cassette is required to achieve expression. |
