| Primary Identifier | MGI:6726096 | Allele Type | Targeted |
| Attribute String | Reporter | Gene | Calca |
| Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6NCrl)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The targeting construct inserted the tdTomato openreading frame into the Calca locus between just 5â² of the initiation codon in exon 2 and the noncoding exon 6. An frt-flanked Sv-Neo gene was also included for positive selection. After homologous recombination in ES cells, resulting chimeras were bred with FLPase mice to remove the Sv-Neo gene. |
