| Primary Identifier | MGI:6755214 | Allele Type | Targeted |
| Attribute String | Inserted expressed sequence, Recombinase | Gene | Cd209b |
| Transmission | Germline | Strain of Origin | C57BL/6 |
| Is Recombinase | true | Is Wild Type | false |
| molecularNote | A cDNA construct encoding an Frt-flanked neomycin resistance cassette followed by a stop cassette was introduced before a start codon, followed by cDNA encoding Cre recombinase, and the human DTR (HBEGF) was generated. The Cre recombinase and DTR coding sequences were separated by self-cleaving 2A viral sequences to allow stoichiometric translation of both individual proteins. This construct was inserted at the ATG start codon of cd209b, effectively replacing the expression of SIGN-R1. The neomycin resistance gene and stop cassette was removed by crossing to FLPO mice. |
