| Primary Identifier | MGI:6727369 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Anks6 |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | KOMP-CSD |
| description | Mutant cell line EPD0109_5_E04 was used to create mutant mice which demonstrated germline transmission (J:307271). |
| molecularNote | The L1L2_Bact_P cassette was inserted at position 47050034 of Chromosome 4 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 2 at position 47050034. The critical exon 2 is thus flanked by loxP sites. Flp-mediated recombination removed the lacZ and neomycin resistance gene. Cre-mediated recombination deleted exon 2 and generated a knockout mouse. Western blot analysis confirmed the absence of protein in mouse embryonic fibroblasts. |
