Primary Identifier | MGI:6827385 | Allele Type | Targeted |
Attribute String | Conditional ready | Gene | Xbp1 |
Transmission | Germline | Strain of Origin | Not Specified |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Using homologous recombination in ES cells, the targeting vector inserted an Frt flanked neomycin cassette downstream of Xbp1 exon 5, two loxP sites flanking the exons 4 and 5 together with the neo-cassette, and a modified version of Xbp1 exon 4 (E4Î26) along with exon 5 was positioned downstream of the second loxP site. The exon E4Î26 is devoid of the intronic sequence of 26 nucleotides. |